Composite

Part:BBa_K4468011:Design

Designed by: Zhichao Li   Group: iGEM22_HUST-China   (2022-09-30)


T7-PmrA- T7-PmrB(FP)-T7 Terminator-PmrC-Oprf-Sitag-FP- T7 Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2858
    Illegal PstI site found at 1619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1619
    Illegal NgoMIV site found at 3293
    Illegal NgoMIV site found at 4127
    Illegal AgeI site found at 1813
    Illegal AgeI site found at 2642
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

nothing


Source

T7 promoter and T7 terminator are from Bacteriophage T7.
PmrA, PmrB and PmrC are from Salmonella bongori.
Oprf is from P. aeruginosa. Sitag is from Escherichia coli. LanM is from Bacteria. dLBT is from Fungus.

References