![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4468011:Design
Designed by: Zhichao Li Group: iGEM22_HUST-China (2022-09-30)
T7-PmrA- T7-PmrB(FP)-T7 Terminator-PmrC-Oprf-Sitag-FP- T7 Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1619
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2858
Illegal PstI site found at 1619 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1138
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1619
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1619
Illegal NgoMIV site found at 3293
Illegal NgoMIV site found at 4127
Illegal AgeI site found at 1813
Illegal AgeI site found at 2642 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
nothing
Source
T7 promoter and T7 terminator are from Bacteriophage T7.
PmrA, PmrB and PmrC are from Salmonella bongori.
Oprf is from P. aeruginosa. Sitag is from Escherichia coli. LanM is from Bacteria. dLBT is from Fungus.